![]() ![]() As a consequence, with 5 and 3 µm particles a higher flow rate will result in a shorter analysis time, however, under the loss of resolution. However, in agreement with the van Deemter equation, the plate height h, after reaching a minimum (maximum plate number), increases with the further increase of the linear velocity u. a lower plate height h at high linear velocity u is required. In order to increase the efficiency of HPLC columns with respect to higher resolution and shorter analysis times, a higher plate number N, i.e. Since the requirements of HPLC separations with respect to the highest efficiency have not yet been met, further optimizations were necessary. While monolithic phases enable the required decrease in analysis times on conventional instruments at low pressures, they do not reach the separation efficiency of sub-2 µm particles. ![]() While a reduction in particle size of totally porous silicas down to sub-2 µm particles results in a distinct shortening of analysis time, the column backpressure sometimes may increase up to 1000 bar, necessitating the costly acquisition of ultra-high pressure LC instrumentation. Modern core-shell technology also meets this demand for high resolution and short analysis at moderate pressures. In the past years, manufacturers of HPLC columns and instruments have made different efforts to develop products with high separation efficiency combined with shorter analysis times and higher sample throughput. CORE-SHELL PHASES – HIGHEST EFFICIENCY FOR ALL HPLC INSTRUMENTS ![]()
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